Mutations in the aph(2")-Ic gene are responsible for increased levels of aminoglycoside resistance.

نویسندگان

  • Hae Kyung Lee
  • Sergei B Vakulenko
  • Don B Clewell
  • Stephen A Lerner
  • Joseph W Chow
چکیده

Random PCR mutagenesis of the enterococcal aph(2")-Ic gene followed by selection for mutant enzymes that confer enhanced levels of aminoglycoside resistance resulted in mutants of APH(2")-Ic with His-258-Leu and Phe-108-Leu substitutions, all of which conferred rises in the MICs of several aminoglycosides. The mutated residues are located outside conserved regions of aminoglycoside phosphotransferases.

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عنوان ژورنال:
  • Antimicrobial agents and chemotherapy

دوره 46 10  شماره 

صفحات  -

تاریخ انتشار 2002